Protein A Antibody

Detection of Protein A by Western Blot.

Western blot shows Recombinant Protein A Long, Recombinant Protein A, and Recombinant Protein G (negative control). The left panel of the PVDF membrane was probed with 2 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein A at approximately 33 kDa and Protein A Long at approximately 55 kDa (as indicated). To show specificity, the right panel was probed with Anti-Rat IgG Antibody (negative control). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Protein A by Simple Western^TM.

Simple Western lane view recombinant Protein A Long, recombinant Protein A, and recombinant Protein G (negative control), loaded at 0.2 mg/mL. A specific band was detected for Protein A at approximately 40 kDa and Protein A Long at approximately 58 kDa (as indicated) using 20 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.