Protein A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB99761
Key Product Details
Species Reactivity
Multi-Species
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 946418
Product Specifications
Immunogen
Recombinant Protein A
Specificity
Detects recombinant Protein A in direct ELISA and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Protein A Antibody
Detection of Protein A by Western Blot.
Western blot shows Recombinant Protein A Long, Recombinant Protein A, and Recombinant Protein G (negative control). The left panel of the PVDF membrane was probed with 2 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein A at approximately 33 kDa and Protein A Long at approximately 55 kDa (as indicated). To show specificity, the right panel was probed with Anti-Rat IgG Antibody (negative control). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Protein A by Simple WesternTM.
Simple Western lane view recombinant Protein A Long, recombinant Protein A, and recombinant Protein G (negative control), loaded at 0.2 mg/mL. A specific band was detected for Protein A at approximately 40 kDa and Protein A Long at approximately 58 kDa (as indicated) using 20 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Protein A Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Recombinant Protein A Long and Recombinant Protein A
Sample: Recombinant Protein A Long and Recombinant Protein A
Western Blot
2 µg/mL
Sample: Recombinant Protein A Long and Recombinant Protein A
Sample: Recombinant Protein A Long and Recombinant Protein A
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protein A
Additional Protein A Products
Product Documents for Protein A Antibody
Product Specific Notices for Protein A Antibody
For research use only
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