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Protein Disulfide Isomerase/P4HB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97470

Novus Biologicals, part of Bio-Techne
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NBP1-97470-0.025mg
NBP1-97470-0.05mg
NBP1-97470-0.2mg

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Guinea Pig, Hamster, Primate, Sheep, Xenopus

Cited:

Human, Mouse, Rat, Porcine, Ovine, Primate

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Purified bovine liver Protein Disulfide Isomerase/P4HB.

Marker

Endoplasmic Reticulum Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470] - Analysis of PDI: Lane 1: MWM, Lane 2: Protein Disulfide Isomerase/P4HB (human), (recombinant) (His-tag) (Prod. No.ADI-SPP-891), Lane 3: HeLa, (cell lysate), Lane 4: Rat Testis Tissue Lysate, Lane 5: Mouse Spleen Tissue Lysate.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470] - Analysis of human spleen tissue stained with Protein Disulfide Isomerase/P4HB, pAb at 10ug/ml.

Applications for Protein Disulfide Isomerase/P4HB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Protein Disulfide Isomerase/P4HB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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