Protein Phosphatase 1 beta Antibody (5O9E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16389

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16389-100ul
NBP3-16389-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5O9E7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Protein Phosphatase 1 beta Antibodies »

Product Summary for Protein Phosphatase 1 beta Antibody (5O9E7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 228-327 of human Protein Phosphatase 1 beta (P62140). ADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protein Phosphatase 1 beta Antibody (5O9E7)

Western Blot: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Western Blot: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Western Blot: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Western blot analysis of extracts of various cell lines, using Protein Phosphatase 1 beta Rabbit mAb (NBP3-16389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Immunocytochemistry/Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunofluorescence analysis of U-2 OS cells using Protein Phosphatase 1 beta Rabbit mAb (NBP3-16389) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunohistochemistry of paraffin-embedded mouse kidney using Protein Phosphatase 1 beta Rabbit mAb (NBP3-16389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Protein Phosphatase 1 beta Antibody (5O9E7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protein Phosphatase 1 beta

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favoring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Alternate Names

EC 3.1.3.16, EC 3.1.3.53, MGC3672, PP1beta, PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform, PPP1CD, protein phosphatase 1, catalytic subunit, beta isoform, protein phosphatase 1, catalytic subunit, beta isozyme, protein phosphatase 1-beta, protein phosphatase 1-delta, serine/threonine protein phosphatase PP1-beta catalytic subunit, serine/threonine-protein phosphatase PP1-beta catalytic subunit

Gene Symbol

PPP1CB

Additional Protein Phosphatase 1 beta Products

Product Documents for Protein Phosphatase 1 beta Antibody (5O9E7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Protein Phosphatase 1 beta Antibody (5O9E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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