Protocadherin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47321

Novus Biologicals, part of Bio-Techne
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NBP2-47321
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Protocadherin-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AKSGYQAGKKETKDLYAPKPSGKASKGNKSKGKKSKSPKPVKPVEDEDEAGLQKSLKFNLMSDAPGDSPRIHLPLNYPP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protocadherin-1 Antibody

Western Blot: Protocadherin-1 Antibody [NBP2-47321]

Western Blot: Protocadherin-1 Antibody [NBP2-47321]

Western Blot: Protocadherin-1 Antibody [NBP2-47321] - Analysis in control (vector only transfected HEK293T lysate) and PCDH1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody [NBP2-47321]

Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody [NBP2-47321]

Immunocytochemistry/Immunofluorescence: Protocadherin-1 Antibody [NBP2-47321] - Staining of human cell line HaCaT shows localization to nucleoli, cell junctions & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Protocadherin-1 Antibody [NBP2-47321]

Immunohistochemistry-Paraffin: Protocadherin-1 Antibody [NBP2-47321]

Immunohistochemistry-Paraffin: Protocadherin-1 Antibody [NBP2-47321] - Staining of human cerebral cortex shows strong cytoplasmic and nucleolar positivity in neuronal cells.

Applications for Protocadherin-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protocadherin-1

PCDH1 belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene.

Alternate Names

PC42, PCDH1, PCDH42, Protocadherin1

Gene Symbol

PCDH1

Additional Protocadherin-1 Products

Product Documents for Protocadherin-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Protocadherin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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