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Prox1 Antibody (5G10) [Alexa Fluor® 647]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30045AF647

Novus Biologicals, part of Bio-Techne
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NBP1-30045AF647

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Prox1 Antibody (5G10) [Alexa Fluor® 647]

Immunogen

The highly conserved Homeo and Prospero domain of human Prox1. [UniProt# Q92786]

Epitope

The homeo prospero domain of Prox1

Reactivity Notes

It is expected to work with all vertebrates including mammals, birds and fish. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Prox1 Antibody (5G10) [Alexa Fluor® 647]

Flow Cytometry: Prox1 Antibody (5G10) [Alexa Fluor® 647] [NBP1-30045AF647] - An intracellular stain was performed on HepG2 cells with Prox1 [5G10] Antibody NBP1-30045AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for Prox1 Antibody (5G10) [Alexa Fluor® 647]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry Whole-Mount

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Prox1

PROX1 may play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons. PROX1 antibodies are also used as a highly specific marker of lymphatic endothelial cells.

Long Name

Prospero-related Homeobox 1

Alternate Names

Homeobox prospero-like protein PROX1, prospero homeobox 1, prospero homeobox protein 1, prospero-related homeobox 1, PROX-1

Gene Symbol

PROX1

Additional Prox1 Products

Product Documents for Prox1 Antibody (5G10) [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Prox1 Antibody (5G10) [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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