Prox1 Antibody (5G10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80920

Novus Biologicals, part of Bio-Techne
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NBP2-80920
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Catalog #
BSA Free
NBP1-30045
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G10

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Prox1 Antibodies »

Product Summary for Prox1 Antibody (5G10) - Azide and BSA Free

Immunogen

The highly conserved Homeo and Prospero domain of human Prox1. [UniProt# Q92786]

Epitope

The homeo prospero domain of Prox1

Reactivity Notes

It is expected to work with all vertebrates including mammals, birds and fish. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Prox1 Antibody (5G10) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]

Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]

Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]

Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot UD1009o of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic stain
Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]

Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]

Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - HepG2 cells were stained with Prox1 (5G10) NBP1-30045 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody. Image from the standard format of this antibody.
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Applications for Prox1 Antibody (5G10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry Whole-Mount

reported in scientific literature (PMID 24654984)

Immunohistochemistry-Paraffin

1:100-1:1000
Application Notes
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Prox1

Prox1 is a prospero related homeobox gene. Prox1 may play a fundamental role in early development of the CNS. It may also regulate gene expression and development of postmitotic undifferentiated young neurons. Prox1 is expressed in CNS, eye, pancreas, liver and heart tissues, and it is one of the most specific and reliable markers for lymphatic endothelial cells. On a subcellular level Prox1 resides in the nucleus.

Long Name

Prospero-related Homeobox 1

Alternate Names

Homeobox prospero-like protein PROX1, prospero homeobox 1, prospero homeobox protein 1, prospero-related homeobox 1, PROX-1

Gene Symbol

PROX1

Additional Prox1 Products

Product Documents for Prox1 Antibody (5G10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Prox1 Antibody (5G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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