Prox1 Antibody (9I9A7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16739
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9I9A7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Prox1 Antibody (9I9A7)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PROX1 (Q92786). MPDHDSTALLSRQTKRRRVDIGVKRTVGTASAFFAKARATFFSAMNPQGSEQDVEYSVVQHADGEKSNVLRKLLKRANSYEDAMMPFPGATIISQLLKNN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Prox1 Antibody (9I9A7)
![Western Blot: Prox1 Antibody (9I9A7) [NBP3-16739]](https://resources.bio-techne.com/images/products/Prox1-Antibody-9I9A7-Western-Blot-NBP3-16739-img0002.jpg "Western Blot: Prox1 Antibody (9I9A7) [NBP3-16739]")
Western Blot: Prox1 Antibody (9I9A7) [NBP3-16739]
Western Blot: Prox1 Antibody (9I9A7) [NBP3-16739] - Western blot analysis of extracts of various cell lines, using Prox1 Rabbit mAb (NBP3-16739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.![Immunohistochemistry-Paraffin: Prox1 Antibody (9I9A7) [NBP3-16739]](https://resources.bio-techne.com/images/products/Prox1-Antibody-9I9A7-Immunohistochemistry-Paraffin-NBP3-16739-img0001.jpg "Immunohistochemistry-Paraffin: Prox1 Antibody (9I9A7) [NBP3-16739]")
Immunohistochemistry-Paraffin: Prox1 Antibody (9I9A7) [NBP3-16739]
Immunohistochemistry-Paraffin: Prox1 Antibody (9I9A7) [NBP3-16739] - Immunohistochemistry of paraffin-embedded human placenta using Prox1 Rabbit mAb (NBP3-16739) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Prox1 Antibody (9I9A7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Prox1
Long Name
Prospero-related Homeobox 1
Alternate Names
Homeobox prospero-like protein PROX1, prospero homeobox 1, prospero homeobox protein 1, prospero-related homeobox 1, PROX-1
Gene Symbol
PROX1
Additional Prox1 Products
Product Documents for Prox1 Antibody (9I9A7)
Product Specific Notices for Prox1 Antibody (9I9A7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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