PSAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33459

Novus Biologicals, part of Bio-Techne
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NBP1-33459
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PSAP Antibodies »

Product Summary for PSAP Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSAP. The exact sequence is proprietary.

Reactivity Notes

Pig (87%), Bovine (87%).

Localization

Lysosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSAP Antibody

Western Blot: PSAP Antibody [NBP1-33459]

Western Blot: PSAP Antibody [NBP1-33459]

Western Blot: PSAP Antibody [NBP1-33459] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-33459]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-33459]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-33459] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: PSAP Antibody [NBP1-33459]

Western Blot: PSAP Antibody [NBP1-33459]

Western Blot: PSAP Antibody [NBP1-33459] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Applications for PSAP Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSAP

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

Long Name

Prosaposin

Alternate Names

GLBA, SAP1

Gene Symbol

PSAP

Additional PSAP Products

Product Documents for PSAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PSAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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