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PSENEN/PEN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-227

Novus Biologicals, part of Bio-Techne
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NB300-227
NB300-227SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

A synthetic peptide corresponding to the C-terminus of human PEN2 (within residues 50-101). [UniProt# Q9NZ42]

Reactivity Notes

Human and mouse neuroblastoma (N2a) cells.

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSENEN/PEN2 Antibody

Western Blot: PSENEN/PEN2 Antibody [NB300-227]

Western Blot: PSENEN/PEN2 Antibody [NB300-227]

Western Blot: PEN2 Antibody [NB300-227] - Analysis of PEN2 on human placenta.

Applications for PSENEN/PEN2 Antibody

Application
Recommended Usage

Immunoprecipitation

1:50-1:100

Western Blot

1:1000-1:2500
Application Notes
This PEN2 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at approximately 12 kDa. This antibody has primarily been tested in HA-PEN2 transfected cells combining Immunoprecipitation with Western blot analysis (ECL). For endogenous sample testing, it is suggested that the investigator load a greater amount of protein on the gel and to combine Immunoprecipitation with Western blot analysis.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSENEN

PEN2 (gamma-secretase subunit PEN-2 or presenilin enhancer protein 2) is an essential subunit of the gamma-secretase complex which is an endoprotease complex responsible for catalyzing the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Gamma-secretase complex is composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2. PEN2 represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity. PEN2 is a multi-pass membrane protein predominantly localized in the endoplasmic reticulum and in the Golgi stack membrane/cis-Golgi. Defects in PEN2 have been linked to familial acne inversa type 2 (ACNINV2), a chronic relapsing inflammatory disease of the hair follicles which is primarily characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars.

Long Name

Presenilin Enhancer 2 Homolog

Alternate Names

PEN-2

Entrez Gene IDs

55851 (Human)

Gene Symbol

PSENEN

UniProt

Additional PSENEN Products

Product Documents for PSENEN/PEN2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSENEN/PEN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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