PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80921

Novus Biologicals, part of Bio-Techne
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NBP2-80921
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, ELISA, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Kappa Clone # HECA-452

Format

Azide and BSA Free

Concentration

1 mg/ml

View all PSGL-1/CD162 Antibodies »

Product Summary for PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free

Immunogen

Human tonsil stroma

Specificity

CLA antibody clone HECA-452 is known to recognize the cutaneous lymphocyte-associated antigen / CLA which is a specialized glycosylated form of P-selectin glycoprotein ligand-1/PSGL-1. Besides lymphocytes, this clone has been shown to react with normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).

Clonality

Monoclonal

Host

Rat

Isotype

IgM Kappa

Scientific Data Images for PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free

Western Blot: PSGL-1/CD162 Antibody (HECA-452)Azide and BSA Free [NBP2-80921]

Western Blot: PSGL-1/CD162 Antibody (HECA-452)Azide and BSA Free [NBP2-80921]

Western Blot: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml. Image from the standard format of this antibody.
Immunohistochemistry: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921]

Immunohistochemistry: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921]

Immunohistochemistry: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast adenocarcinoma using CLA antibody (clone HECA-452) at 5 ug/ml concentration. The antibody generated a membrane-cytoplasmic staining in the cancerous cells of breast adenocarci
Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921]

Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921]

Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems]. A co-stain was performed using CD3 antibody NBP2-25186APC. Image from the standard format of this antibody.
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Applications for PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/10^6 cells in 100 ul

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

2 ug/ml
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSGL-1/CD162

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Gene Symbol

SELPLG

Additional PSGL-1/CD162 Products

Product Documents for PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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