PSP94/MSMB Antibody (4L8B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16346

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16346-100ul
NBP3-16346-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4L8B5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PSP94/MSMB Antibodies »

Product Summary for PSP94/MSMB Antibody (4L8B5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PSP94/MSMB (P08118). MNVLLGSVVIFATFVTLCNASCYFIPNEGVPGDSTRKCMDLKGNKHPINSEWQTDNCETCTCYETEISCCTLVSTPVGYDKDNCQRIFKKEDCKYIVVEK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSP94/MSMB Antibody (4L8B5)

Western Blot: PSP94/MSMB Antibody (4L8B5) [NBP3-16346]

Western Blot: PSP94/MSMB Antibody (4L8B5) [NBP3-16346]

Western Blot: PSP94/MSMB Antibody (4L8B5) [NBP3-16346] - Western blot analysis of extracts of various cell lines, using PSP94/MSMB Rabbit mAb (NBP3-16346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: PSP94/MSMB Antibody (4L8B5) [NBP3-16346]

Immunocytochemistry/ Immunofluorescence: PSP94/MSMB Antibody (4L8B5) [NBP3-16346]

Immunocytochemistry/Immunofluorescence: PSP94/MSMB Antibody (4L8B5) [NBP3-16346] - Immunofluorescence analysis of U-2 OS cells using PSP94/MSMB Rabbit mAb (NBP3-16346) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for PSP94/MSMB Antibody (4L8B5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSP94/MSMB

PSP94 (prostate secretory protein of 94 amino acids; also named beta-MSP) is a secreted, non-glycosylated member of the beta-microseminoprotein family. The 94 amino acid (aa) mature PSP94 contains no classic motifs or domains, but does have ten Cys that are conserved across species. It is expressed in mucoid secretions, but its function is unknown. PSP94 is abundant in prostatic fluid, which is the exclusive source in rodents. Gastric and respiratory secretory epithelia are also significant sources in humans. Human PSP94 circulates bound to a 71 kDa PSP binding protein, possibly disulfide-linked. The seminal fluid protein CRISP-3 can also bind PSP94. PSP94 has been proposed as an alternative to PSA as a serum marker for prostate cancer. When total (bound plus free) PSP94 is considered, its secretion is found to be down-regulated in cancer cells, creating below normal circulating levels. Its size is predicted at 11 kDa, but may appear to be 16 kDa due to anomalous migration. A 61 aa variant, formed by C-terminal proteolysis, is increased in prostate secretions from patients with benign prostatic hyperplasia. A prostate-specific alternate splice form shows a substitution of 41 aa for the C-terminal 78 aa. Mature human PSP94 shares 53%, 46%, 43%, and 42% aa identity with porcine, rat, mouse, and chicken PSP94, respectively. Most of the ten primate sequences available show less than 80% aa identity. PSP94 has been proposed as a species barrier protein due to its low evolutionary conservation and abundance in seminal fluid.

Long Name

Prostate Secretory Protein of 94 Amino Acids/Microseminoprotein, beta

Alternate Names

IGBF, MSMB, MSPB, PIP, PN44, PRPS, PSP57

Gene Symbol

MSMB

Additional PSP94/MSMB Products

Product Documents for PSP94/MSMB Antibody (4L8B5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PSP94/MSMB Antibody (4L8B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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