PTBP2 Antibody (10S9P6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16756
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10S9P6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP2 (Q9UKA9). MDGIVTEVAVGVKRGSDELLSGSVLSSPNSNMSSMVVTANGNDSKKFKGEDKMDGAPSRVLHIRKLPGEVTETEVIALGLPFGKVTNILMLKGKNQAFLE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PTBP2 Antibody (10S9P6)
![Western Blot: PTBP2 Antibody (10S9P6) [NBP3-16756]](https://resources.bio-techne.com/images/products/PTBP2-Antibody-10S9P6-Western-Blot-NBP3-16756-img0005.jpg "Western Blot: PTBP2 Antibody (10S9P6) [NBP3-16756]")
Western Blot: PTBP2 Antibody (10S9P6) [NBP3-16756]
Western Blot: PTBP2 Antibody (10S9P6) [NBP3-16756] - Western blot analysis of extracts of various cell lines, using PTBP2 Rabbit mAb (NBP3-16756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: PTBP2 Antibody (10S9P6) [NBP3-16756] -
Immunocytochemistry/ Immunofluorescence: PTBP2 Antibody (10S9P6) [NBP3-16756] - Confocal imaging of U-2 OS cells using PTBP2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunohistochemistry: PTBP2 Antibody (10S9P6) [NBP3-16756] -
Immunohistochemistry: PTBP2 Antibody (10S9P6) [NBP3-16756] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PTBP2 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Applications for PTBP2 Antibody (10S9P6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PTBP2
Alternate Names
brPTB, FLJ34897, neural polypyrimidine tract binding protein, Neural polypyrimidine tract-binding protein, Neurally-enriched homolog of PTB, nPTB, nPTB6, nPTB7, nPTB8, polypyrimidine tract binding protein 2, polypyrimidine tract-binding protein 2, PTB-like protein, PTBPTBLPnPTB5, splicing regulator
Gene Symbol
PTBP2
Additional PTBP2 Products
Product Documents for PTBP2 Antibody (10S9P6)
Product Specific Notices for PTBP2 Antibody (10S9P6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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