Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI5C12

Concentration

1 mg/ml

View all PTK9 Antibodies »

Product Summary for PTK9 Antibody (OTI5C12)

Immunogen

Human recombinant protein fragment corresponding to amino acids 140-384 of human TWF1(NP_002813) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

43.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTK9 Antibody (OTI5C12)

Western Blot: PTK9 Antibody (OTI5C12) [NBP2-46251]

Western Blot: PTK9 Antibody (OTI5C12) [NBP2-46251]

Western Blot: PTK9 Antibody (OTI5C12) [NBP2-46251] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and TWF1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-TWF1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunohistochemistry-Paraffin: PTK9 Antibody (OTI5C12) [NBP2-46251]

Immunohistochemistry-Paraffin: PTK9 Antibody (OTI5C12) [NBP2-46251]

Immunohistochemistry-Paraffin: PTK9 Antibody (OTI5C12) [NBP2-46251] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TWF1 mouse monoclonal antibody.
Western Blot: PTK9 Antibody (OTI5C12) [NBP2-46251]

Western Blot: PTK9 Antibody (OTI5C12) [NBP2-46251]

Western Blot: PTK9 Antibody (OTI5C12) [NBP2-46251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1.
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Applications for PTK9 Antibody (OTI5C12)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTK9

PTK9 encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.

Alternate Names

A6, A6 protein tyrosine kinase, Protein A6, Protein tyrosine kinase 9MGC23788, PTK9 protein tyrosine kinase 9, PTK9MGC41876, twinfilin, actin-binding protein, homolog 1 (Drosophila), twinfilin-1

Gene Symbol

TWF1

Additional PTK9 Products

Product Documents for PTK9 Antibody (OTI5C12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTK9 Antibody (OTI5C12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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