The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Gene Symbol

PTPN1

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Product Documents for PTP1B/PTPN1 Antibody (226) [Alexa Fluor® 647]

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Product Specific Notices for PTP1B/PTPN1 Antibody (226) [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PTP1B/PTPN1 Antibody (226) [Alexa Fluor® 647]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89400AF647

Key Product Details

Species Reactivity

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Product Summary for PTP1B/PTPN1 Antibody (226) [Alexa Fluor® 647]

Immunogen

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Isotype

Applications for PTP1B/PTPN1 Antibody (226) [Alexa Fluor® 647]

ELISA

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

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Shipping

Stability & Storage

Background: PTP1B