PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73729
![Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73729-img0008.jpg "Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]")
![Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Azide-and-BSA-Free-Western-Blot-NBP2-73729-img0010.jpg "Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Rat, Canine, Monkey, Primate
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI1D10
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free
Immunogen
Full length human recombinant protein of human PTPN1(NP_002818) produced in HEK293 cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free
Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]
Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.![Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73729-img0007.jpg "Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]")
Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]
Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1.![Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-73729-img0006.jpg "Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]")
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.![Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Azide-and-BSA-Free-Western-Blot-NBP2-73729-img0001.jpg "Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]")
![Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-73729-img0004.jpg "Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]")
![Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-73729-img0005.jpg "Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]")
![Knockout Validated: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Azide-and-BSA-Free-Knockout-Validated-NBP2-73729-img0003.jpg "Knockout Validated: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]")
Applications for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PTP1B
Long Name
Protein Tyrosine Phosphatase 1B
Alternate Names
PTPN1
Gene Symbol
PTPN1
Additional PTP1B Products
Product Documents for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free
Product Specific Notices for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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