Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunoprecipitation, Western Blot (Negative)

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PTP1B Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human protein tyrosine phosphatase 1B using the numbering given in entry NP_002818.1 (GeneID 5770).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTP1B/PTPN1 Antibody

Immunoprecipitation: PTP1B/PTPN1 Antibody [NBP1-28691]

Immunoprecipitation: PTP1B/PTPN1 Antibody [NBP1-28691]

Immunoprecipitation: PTP1B/PTPN1 Antibody [NBP1-28691] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTPN1 antibody NBP1-28691 used for IP at 10 ug/mg lysate. PTPN1 was also immunoprecipitated by rabbit anti-PTPN1 antibody NBP1-28692, which recognizes a downstream epitope. For blotting immunoprecipitated PTPN1, NBP1-28691 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for PTP1B/PTPN1 Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Reviewed Applications

Read 1 review rated 5 using NBP1-28691 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PTP1B

Protein-tyrosine phosphatases 1B (PTP1B) is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTP1B has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Entrez Gene IDs

5770 (Human)

Gene Symbol

PTPN1

UniProt

NP_002818.1 (Human)

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PTP1B/PTPN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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