PTPN13/PTPL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56139

Novus Biologicals, part of Bio-Techne
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NB100-56139
NB100-56139SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all PTPN13/PTPL1 Antibodies »

Product Specifications

Immunogen

A recombinant protein corresponding to amino acids 1279 to 1883 of human FAP-1 protein was used as immunogen; GenBank no. NP_542414.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTPN13/PTPL1 Antibody

Western Blot: PTPN13/PTPL1 Antibody [NB100-56139]

Western Blot: PTPN13/PTPL1 Antibody [NB100-56139]

Western Blot: PTPN13/PTPL1 Antibody - Unpurified [NB100-56139] - PTPN13/PTPL1 Antibody [NB100-56139] - Analysis of PTPN13/PTPL1 using this antibody. In ovarian carcinoma cell lines PTPN13/PTPL1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56139]

Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56139]

Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody - Unpurified [NB100-56139] - PTPN13/PTPL1 Antibody [NB100-56139] - Analysis of PTPN13/PTPL1 in cell lines using this antibody at 1:2000. In ovarian carcinoma cell lines PTPN13/PTPL1, expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively.
Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56139]

Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56139]

Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody - Unpurified [NB100-56139] - PTPN13/PTPL1 Antibody [NB100-56139] - Ovarian carcinoma cores from a tissue microarray at 1:2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-eosin counterstain.

Applications for PTPN13/PTPL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:3000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
FAP-1 typically migrates at ~270 kDa on western blots. Thus for SDS-PAGE and western blot, the researcher should use techniques optimized for the transfer of proteins with high molecular weights. The actual molecular weight of FAP-1 observed depends on the isoform expression.

Reviewed Applications

Read 1 review rated 4 using NB100-56139 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Neat whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTPN13/PTPL1

FAP-1 (also known as Fas-associated phosphatase-1, PTPN13, PTP-BAS, hPTPIE, and PTPL1) is a member of the protein tyrosine phosphatase (PTP) family (reviewed in Meinhold-Heerlein et al, 2001; Savaskan et al, 2005; Foehr et al, 2005; and Ivanov et al, 2006). PTPs are enzymes that catalyze the removal of a phosphate group attached to a tyrosine residue. Most intracellular signaling involves reversible phosphorylation events; therefore, PTPs are central to the dynamic regulation of signaling cascades that underlie cell functions. For example, PTPs play key roles in regulating cell growth, differentiation, proliferation, inflammation, and oncogenic transformation. PTPs are emerging as a promising class of signaling targets for diseases such as cancer, neurodegeneration, diabetes, and inflammation. A key challenge is to identify specific PTPs that are involved in a disease process and develop therapeutics to modulate the PTP. FAP-1 phosphatase is thought to be important in the Fas signaling pathway. FAP-1 binds to the cytosolic tail of the Fas receptor (Apo1, CD95) and inhibits Fas-induced apoptosis. Increased FAP-1 protein levels in some tumor cell lines and tumor tissues correlates with resistance to Fas-mediated apoptosis. In general, FAP-1 expression has been found to be highest in cell lines and tissues that are relatively resistant to Fas-mediated apoptosis. Gene transfer-mediated elevations in FAP-1 partially abolished Fas-induced apoptosis in a T cell line which is consistent with an inhibitory effect of FAP-1 on Fas signal transductions. Additionally, FAP-1 expression correlates with Fas resistant in ovarian cancer cell lines and FAP-1 is commonly expression in ovarian cancers. Human Fas has a putative consensus tyrosine phosphorylation site (Tyrosine 275) suggesting that Fas surface expression or signaling may be regulated by phosphorylation. FAP-1 has been shown to directly bind to Fas and may dephosphorylate Fas as part of the down regulation of the apoptotic pathway. It is thought that development of therapeutics to inhibit FAP-1 may increase the ability of tumor cells with upregulated FAP-1 to undergo apoptosis. FAP-1 is a large approx. 270 kDa protein. Multiple alternatively spliced FAP-1 transcript variants which encode distinct proteins have been reported, including shorter forms. Please see Application Notes section for additional details on FAP-1 isoforms.

Long Name

Protein Tyrosine Phosphatase, Non-receptor Type 13

Alternate Names

EAP-1, FAP-1, PNP1, PTP-BAS, PTP-BL, PTP1E, PTPL1, PTPLE

Entrez Gene IDs

5783 (Human)

Gene Symbol

PTPN13

UniProt

Q12923

Additional PTPN13/PTPL1 Products

Product Documents for PTPN13/PTPL1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PTPN13/PTPL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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