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PTRF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80220

Novus Biologicals, part of Bio-Techne
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NBP1-80220

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide directed towards the middle region of mouse PTRF. Peptide sequence SEALPEKEGDELGEGERPEDDTAAIELSSDEAVEVEEVIEESRAERIKRS. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PTRF Antibody

Western Blot: PTRF Antibody [NBP1-80220]

Western Blot: PTRF Antibody [NBP1-80220]

Western Blot: PTRF Antibody [NBP1-80220] - Sample Tissue: Mouse Lung Antibody Dilution: 1.0ug/ml
Immunohistochemistry-Paraffin: PTRF Antibody [NBP1-80220]

Immunohistochemistry-Paraffin: PTRF Antibody [NBP1-80220]

Immunohistochemistry-Paraffin: PTRF Antibody [NBP1-80220] - Mouse vessel and adipocytes (skeletal muscle), 5 ug/ml.
Western Blot: PTRF Antibody [NBP1-80220]

Western Blot: PTRF Antibody [NBP1-80220]

Western Blot: PTRF Antibody [NBP1-80220] - NIH/3T3 cell lysate, Antibody Titration: 0.625ug/ml

Applications for PTRF Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTRF

PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13.

Alternate Names

CAVIN, Cavin-1, CGL4, FLJ90031, polymerase I and transcript release factor, RNA polymerase I and transcript release factor, TTF-I interacting peptide 12

Gene Symbol

CAVIN1

Additional PTRF Products

Product Documents for PTRF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PTRF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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