PU.1/Spi-1 Antibody (PU1/2146)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75738

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PU1/2146

Concentration

0.2 mg/ml

View all PU.1/Spi-1 Antibodies »

Product Summary for PU.1/Spi-1 Antibody (PU1/2146)

Immunogen

Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 )

Localization

Nuclear

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75766)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PU.1/Spi-1 Antibody (PU1/2146)

Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of THP-1 cell lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).
Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1/Spi-1 Antibody (PU1/2146) followed by Goat anti-Mouse IgG-CF488 (Green).
Membrane is labeled with Phalloidin (Red).
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
View 5 more images

Applications for PU.1/Spi-1 Antibody (PU1/2146)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

UniProt

P17947 (Human)

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody (PU1/2146)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PU.1/Spi-1 Antibody (PU1/2146)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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