PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 500 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75766MFV500

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PU1/2146

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PU.1/Spi-1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 )

Localization

Nuclear

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 500 SE]

PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 500 SE]

PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 500 SE] -

PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.

Applications for PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 500 SE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 500 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 500 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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