PU.1/Spi-1 Antibody (S02-1D7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19911

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19911-100ul
NBP3-19911-50ul
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-1D7

Concentration

0.3 mg/ml

View all PU.1/Spi-1 Antibodies »

Product Summary for PU.1/Spi-1 Antibody (S02-1D7)

Immunogen

A synthetic peptide of mouse PU.1/Spi1 (Uniprot # P17433)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PU.1/Spi-1 Antibody (S02-1D7)

Western Blot: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911]

Western Blot: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911]

Western Blot: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911] - Western blot detection of PU.1/Spi1 in CHO-K1, C6 cell lysates using NBP3-19911 (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 31kDa.
Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911]

Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911]

Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911] - Immunocytochemistry of PU.1/Spi1 (green) in Raw264. 7 using NBP3-19911 at dilution 1/50, and DAPI (blue)

Applications for PU.1/Spi-1 Antibody (S02-1D7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody (S02-1D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PU.1/Spi-1 Antibody (S02-1D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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