PU.1/Spi-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33427

Novus Biologicals, part of Bio-Techne
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NBP2-33427
NBP2-33427-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PU.1/Spi-1 Antibodies »

Product Summary for PU.1/Spi-1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PU.1/Spi-1 Antibody

Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody [NBP2-33427]

Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody [NBP2-33427]

Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human cell line REH shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427] - Staining in human bone marrow and pancreas tissues using anti-SPI1 antibody. Corresponding SPI1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human bone marrow shows high expression.
View 1 more images

Applications for PU.1/Spi-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

UniProt

P17947 (Human)

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PU.1/Spi-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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