PUF60 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49906
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Summary for PUF60 Antibody
Immunogen
The immunogen for this product maps to a region between residue 509 and 559 of human Poly-U Binding Splicing Factor 60KDa using the numbering given in entry NP_510965.1 (GeneID 22827).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PUF60 Antibody
![Western Blot: PUF60 Antibody [NBP1-49906]](https://resources.bio-techne.com/images/products/PUF60-Antibody-Western-Blot-NBP1-49906-img0001.jpg "Western Blot: PUF60 Antibody [NBP1-49906]")
Western Blot: PUF60 Antibody [NBP1-49906]
Western Blot: PUF60 Antibody [NBP1-49906] - Detection of Human PUF60 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize upstream epitopes. For blotting immunoprecipitated PUF60 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-49906]](https://resources.bio-techne.com/images/products/PUF60-Antibody-Immunohistochemistry-NBP1-49906-img0003.jpg "Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-49906]")
Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-49906]
Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-49906] - FFPE section of human prostate carcinoma (left) and mouse teratoma (right).Antibody: Affinity purified rabbit anti-PUF60 used at a dilution of 1:200 (1ug/ml)Detection: DABApplications for PUF60 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PUF60
Alternate Names
FBP interacting repressor, FBP-interacting repressor, FIRpoly-U binding splicing factor PUF60, FLJ31379, FUSE-binding protein-interacting repressor, poly(U)-binding-splicing factor PUF60, poly-U binding splicing factor 60KDa, Ro ribonucleoprotein binding protein 1, Ro ribonucleoprotein-binding protein 1, Ro-binding protein 1, roBP1, ROBPI, siah binding protein 1,60 kDa poly(U)-binding-splicing factor, Siah-binding protein 1, Siah-BP1, SIAHBP1pyrimidine tract binding splicing factor
Gene Symbol
PUF60
Additional PUF60 Products
Product Documents for PUF60 Antibody
Product Specific Notices for PUF60 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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