Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Gel Supershift Assay, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all PUM1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 225 and 275 of human Pumilio 1 using the numbering given in entry NP_055491.1 (GeneID 9698).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUM1 Antibody

Western Blot: PUM1 Antibody [NB100-259]

Western Blot: PUM1 Antibody [NB100-259]

Western Blot: PUM1 Antibody [NB100-259] - Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody used for WB at 0.1 ug/ml. Secondary: HRPconjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.
Western Blot: PUM1 Antibody [NB100-259]

Western Blot: PUM1 Antibody [NB100-259]

Western Blot: PUM1 Antibody [NB100-259] - Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody NB100-259 used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: PUM1 Antibody [NB100-259]

Immunoprecipitation: PUM1 Antibody [NB100-259]

Immunoprecipitation: PUM1 Antibody [NB100-259] - Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293Tcells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-Pumillio 1 antibody NB100-259 (lot NB100-259-3) used for IP at 6 ug per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-259-2) and another rabbit anti-Pumillio 1 antibody. For blotting immunoprecipitated Pumillio 1, NB100-259 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 seconds.

Applications for PUM1 Antibody

Application
Recommended Usage

Gel Super Shift Assays

1:1-1:100

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Gel Super Shift Assays and Immunoprecipitation were reported in scientific literature (PMID: 23071749). Use in ICC/IF was reported in scientific literature (PMID: 23071749).

Reviewed Applications

Read 1 review rated 5 using NB100-259 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PUM1

This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Long Name

Pumilio 1

Alternate Names

HSPUM, PUMH1, Pumilio-1, PUML1

Entrez Gene IDs

9698 (Human); 80912 (Mouse)

Gene Symbol

PUM1

UniProt

Q14671 (Human)

Additional PUM1 Products

Product Documents for PUM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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