PUS7 Antibody (OTI5F12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03112
![Western Blot: PUS7 Antibody (OTI5F12) [NBP2-03112]](https://resources.bio-techne.com/images/products/PUS7-Antibody-5F12-Western-Blot-NBP2-03112-img0003.jpg "Western Blot: PUS7 Antibody (OTI5F12) [NBP2-03112]")
![Flow Cytometry: PUS7 Antibody (OTI5F12) [NBP2-03112]](https://resources.bio-techne.com/images/products/PUS7-Antibody-5F12-Flow-Cytometry-NBP2-03112-img0001.jpg "Flow Cytometry: PUS7 Antibody (OTI5F12) [NBP2-03112]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey, Primate
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI5F12
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PUS7 (NP_061915) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
74.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PUS7 Antibody (OTI5F12)
Western Blot: PUS7 Antibody (5F12) [NBP2-03112] Analysis of extracts (35ug) from 9 different cell lines by using anti-PUS7 monoclonal antibody.
![Immunocytochemistry/ Immunofluorescence: PUS7 Antibody (OTI5F12) [NBP2-03112]](https://resources.bio-techne.com/images/products/PUS7-Antibody-5F12-Immunocytochemistry-Immunofluorescence-NBP2-03112-img0004.jpg "Immunocytochemistry/ Immunofluorescence: PUS7 Antibody (OTI5F12) [NBP2-03112]")
Immunocytochemistry/ Immunofluorescence: PUS7 Antibody (OTI5F12) [NBP2-03112]
Immunocytochemistry/Immunofluorescence: PUS7 Antibody (5F12) [NBP2-03112] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PUS7.![Flow Cytometry: PUS7 Antibody (OTI5F12) [NBP2-03112]](https://resources.bio-techne.com/images/products/PUS7-Antibody-5F12-Flow-Cytometry-NBP2-03112-img0002.jpg "Flow Cytometry: PUS7 Antibody (OTI5F12) [NBP2-03112]")
Flow Cytometry: PUS7 Antibody (OTI5F12) [NBP2-03112]
Flow Cytometry: PUS7 Antibody (5F12) [NBP2-03112] - Analysis of Jurkat cells, using anti-PUS7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for PUS7 Antibody (OTI5F12)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PUS7
Alternate Names
EC 5.4.99.-, FLJ20485, KIAA1897, MGC17720, pseudouridylate synthase 7 homolog, pseudouridylate synthase 7 homolog (S. cerevisiae)
Entrez Gene IDs
54517 (Human)
Gene Symbol
PUS7
Additional PUS7 Products
Product Documents for PUS7 Antibody (OTI5F12)
Product Specific Notices for PUS7 Antibody (OTI5F12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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