PUS7 Antibody (OTI5F12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73733

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5F12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PUS7 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PUS7 (NP_061915) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

74.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUS7 Antibody (OTI5F12) - Azide and BSA Free

Western Blot: PUS7 Antibody (OTI5F12)Azide and BSA Free [NBP2-73733]

Western Blot: PUS7 Antibody (OTI5F12)Azide and BSA Free [NBP2-73733]

Western Blot: PUS7 Antibody (OTI5F12) - Azide and BSA Free [NBP2-73733] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PUS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7.
Immunocytochemistry/ Immunofluorescence: PUS7 Antibody (OTI5F12) - Azide and BSA Free [NBP2-73733]

Immunocytochemistry/ Immunofluorescence: PUS7 Antibody (OTI5F12) - Azide and BSA Free [NBP2-73733]

Immunocytochemistry/Immunofluorescence: PUS7 Antibody (OTI5F12) - Azide and BSA Free [NBP2-73733] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PUS7.
Flow Cytometry: PUS7 Antibody (OTI5F12) - Azide and BSA Free [NBP2-73733]

Flow Cytometry: PUS7 Antibody (OTI5F12) - Azide and BSA Free [NBP2-73733]

Flow Cytometry: PUS7 Antibody (OTI5F12) - Azide and BSA Free [NBP2-73733] - Analysis of Jurkat cells, using anti-PUS7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for PUS7 Antibody (OTI5F12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PUS7

Alternate Names

EC 5.4.99.-, FLJ20485, KIAA1897, MGC17720, pseudouridylate synthase 7 homolog, pseudouridylate synthase 7 homolog (S. cerevisiae)

Gene Symbol

PUS7

Additional PUS7 Products

Product Documents for PUS7 Antibody (OTI5F12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUS7 Antibody (OTI5F12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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