Pygopus-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46171
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Zebrafish
Cited:
Mouse, Fish - Danio rerio (Zebrafish)
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Chemotaxis, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1 and 50 of human Pygopus 2 using the numbering given in entry NP_612157.1 (GeneID 90780).
Reactivity Notes
Zebrafish reactivity reported in scientific literature (PMID: 30366904).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Pygopus-2 Antibody
![Western Blot: Pygopus-2 Antibody [NBP1-46171]](https://resources.bio-techne.com/images/products/Pygopus-2-Antibody-Western-Blot-NBP1-46171-img0004.jpg "Western Blot: Pygopus-2 Antibody [NBP1-46171]")
Western Blot: Pygopus-2 Antibody [NBP1-46171]
Western Blot: Pygopus-2 Antibody [NBP1-46171] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-Pygo2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Pygo2 was also immunoprecipitated by rabbit anti-Pygo2 antibody NBP1-46172, which recognizes a downstream epitope.![Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP1-46171]](https://resources.bio-techne.com/images/products/Pygopus-2-Antibody-Immunohistochemistry-Paraffin-NBP1-46171-img0006.jpg "Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP1-46171]")
Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP1-46171]
Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP1-46171] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Pygo2 used at a dilution of 1:200 (1ug/ml) Detection: DABApplications for Pygopus-2 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Reported in scientific publication (PMID: 23637336)
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Pygopus-2
Additional Pygopus-2 Products
Product Documents for Pygopus-2 Antibody
Product Specific Notices for Pygopus-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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