Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Pygopus-2 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 356 and 406 of human Pygopus 2 using the numbering given in entry NP_612157.1 (GeneID 90780).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pygopus-2 Antibody

Immunohistochemistry: Pygopus-2 Antibody [NBP1-46172]

Immunohistochemistry: Pygopus-2 Antibody [NBP1-46172]

Immunohistochemistry: Pygopus-2 Antibody [NBP1-46172] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Pygo2 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .
Immunoprecipitation: Pygopus-2 Antibody [NBP1-46172]

Immunoprecipitation: Pygopus-2 Antibody [NBP1-46172]

Immunoprecipitation: Pygopus-2 Antibody [NBP1-46172] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Pygo2 antibody used for IP at 3 mcg/mg lysate. Pygo2 was also immunoprecipitated by rabbit anti-Pygo2 antibody NBP1-46171, which recognizes an upstream epitope.

Applications for Pygopus-2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pygopus-2

Pygopus 2 is involved in signal transduction through the Wnt pathway

Alternate Names

PYGO2, Pygopus2

Gene Symbol

PYGO2

Additional Pygopus-2 Products

Product Documents for Pygopus-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pygopus-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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