Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95253

Novus Biologicals, part of Bio-Techne
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NBP2-95253-0.02ml
NBP2-95253-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase E1-alpha subunit Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1). FANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free [NBP2-95253]

Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free [NBP2-95253]

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - Analysis of U-2 OS cells using Pyruvate Dehydrogenase E1-alpha subunit at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free [NBP2-95253]

Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free [NBP2-95253]

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - Analysis of NIH-3T3 cells using Pyruvate Dehydrogenase E1-alpha subunit at dilution of 1:100. Blue: DAPI for nuclear staining.
Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] -

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.
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Applications for Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:20-1:50

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E1-alpha subunit

Pyruvate Dehydrogenase E1-alpha subunit (PDHE1 alpha) is a member of the pyruvate dehydrogenase complex (PDC) and is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293, which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.

Alternate Names

EC 1.2.4.1, PDHA, PDHE1-A type I, PHE1APDHCE1A, pyruvate dehydrogenase (lipoamide) alpha 1, pyruvate dehydrogenase complex, E1-alpha polypeptide 1, pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Gene Symbol

PDHA1

Additional Pyruvate Dehydrogenase E1-alpha subunit Products

Product Documents for Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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