Pyruvate Dehydrogenase E1-alpha subunit Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95269

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-95269
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase E1-alpha subunit Antibodies »

Product Specifications

Immunogen

Recombinant protein of human PDHA1

Reactivity Notes

Predicted cross-reactivities: Mouse, Rat

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyruvate Dehydrogenase E1-alpha subunit Antibody

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95269]

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95269]

Western Blot: PDHA2 Antibody [NBP2-95269] - Western blot analysis was performed by loading 25 ug of various whole cell lysates probed with Rabbit anti PDHA1 (NBP2-95269) at 1:1000 dilution. Arrow points to PDHA1 (molecular weight 43 kDa).
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95269]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95269]

Immunohistochemistry-Paraffin: PDHA2 Antibody [NBP2-95269] - Immunhistochemistry microscopy image of paraffin embedded human mammary cancer stained with Rabbit anti PDHA1 (NBP2-95269) at 1:200 dilution.
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95269]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95269]

Immunohistochemistry-Paraffin: PDHA2 Antibody [NBP2-95269] - Immunohistochemistry microscopy image of paraffin embedded human lung cancer stained with Rabbit anti PDHA1 (NBP2-95269) at 1:200 dilution.

Applications for Pyruvate Dehydrogenase E1-alpha subunit Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E1-alpha subunit

Pyruvate Dehydrogenase E1-alpha subunit (PDHE1 alpha) is a member of the pyruvate dehydrogenase complex (PDC) and is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293, which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.

Alternate Names

EC 1.2.4.1, PDHA, PDHE1-A type I, PHE1APDHCE1A, pyruvate dehydrogenase (lipoamide) alpha 1, pyruvate dehydrogenase complex, E1-alpha polypeptide 1, pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Gene Symbol

PDHA1

UniProt

P08559 (Human)

Additional Pyruvate Dehydrogenase E1-alpha subunit Products

Product Documents for Pyruvate Dehydrogenase E1-alpha subunit Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Pyruvate Dehydrogenase E1-alpha subunit Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.