Pyruvate Dehydrogenase E2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34065

Novus Biologicals, part of Bio-Techne
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NBP2-34065
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase E2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Analysis using Anti-DLAT antibody NBP2-34065 (A) shows similar pattern to independent antibody NBP2-56744 (B).
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065]

Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065]

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human liver shows low expression as expected
View 2 more images

Applications for Pyruvate Dehydrogenase E2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E2

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Alternate Names

70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial

Gene Symbol

DLAT

UniProt

P10515 (Human)

Additional Pyruvate Dehydrogenase E2 Products

Product Documents for Pyruvate Dehydrogenase E2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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