Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67032
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JA67-30
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Pyruvate Dehydrogenase Kinase 1/PDK1 aa 16-65 / 436. (SwissProt: Q15118 Human; SwissProt: Q8BFP9 Mouse; SwissProt: Q63065 Rat)
Localization
Mitochondrion matrix.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Western blot analysis of Pyruvate Dehydrogenase Kinase 1/PDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysateImmunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]
Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Staining PDK1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of paraffin-embedded mouse heart tissue using anti-PDK1 antibody. Counter stained with hematoxylin.Applications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase Kinase 1/PDK1
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.
Alternate Names
EC 2.7.11, EC 2.7.11.2, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, PDH kinase 1, PDK1, PDK-1, PKB kinase, Pyruvate dehydrogenase kinase isoform 1, pyruvate dehydrogenase kinase, isoenzyme 1, pyruvate dehydrogenase kinase, isozyme 1, Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial
Gene Symbol
PDK1
Additional Pyruvate Dehydrogenase Kinase 1/PDK1 Products
Product Documents for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)
Product Specific Notices for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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