Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2383
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]](https://resources.bio-techne.com/images/products/PDK-1-Antibody-Western-Blot-NB100-2383-img0009.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Chicken (93%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]
Reactivity Notes
Predicted to react with mouse and rat based on 100% sequence homology. Immunogen displays the following percentage of sequence identity for non-tested species: chicken (93%).
Localization
Mitochondrion
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]
Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]](https://resources.bio-techne.com/images/products/PDK-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2383-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]")
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]
Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]](https://resources.bio-techne.com/images/products/PDK-1-Antibody-Western-Blot-NB100-2383-img0008.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]")
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2383]
Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.Applications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000-1:2000
Western Blot
2 ug/ml
Application Notes
By Western blot a band is seen at ~49 kDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. In ICC/IF, mitochondiral staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NB100-2383 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase Kinase 1/PDK1
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.
Alternate Names
EC 2.7.11, EC 2.7.11.2, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, PDH kinase 1, PDK1, PDK-1, PKB kinase, Pyruvate dehydrogenase kinase isoform 1, pyruvate dehydrogenase kinase, isoenzyme 1, pyruvate dehydrogenase kinase, isozyme 1, Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial
Gene Symbol
PDK1
Additional Pyruvate Dehydrogenase Kinase 1/PDK1 Products
Product Documents for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Product Specific Notices for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...