Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2384
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2384]](https://resources.bio-techne.com/images/products/PDK-1-Antibody-Western-Blot-NB100-2384-img0005.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2384]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Chicken (92%), Porcine (91%), Primate (100%), Rat (100%), Zebrafish (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoblotting, Western Blot
Cited:
Immunoblotting, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human PDK1 protein sequence (between residues 300-400). [Swiss-Prot Q15118]
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25925251).
Localization
Mitochondrion
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NB100-2384]
Western Blot: PDK-1 Antibody [NB100-2384] - Detection of PDK-1 in human heart lysate using NB 100-2384 at 0.5 ug/ml.Applications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Application
Recommended Usage
Immunoblotting
reported in scientific literature (PMID 28366875)
Western Blot
0.5-2 ug/ml
Application Notes
This PDK1 antibody is useful for Western blot, where a band is seen at ~49 kDa representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase Kinase 1/PDK1
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.
Alternate Names
EC 2.7.11, EC 2.7.11.2, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, PDH kinase 1, PDK1, PDK-1, PKB kinase, Pyruvate dehydrogenase kinase isoform 1, pyruvate dehydrogenase kinase, isoenzyme 1, pyruvate dehydrogenase kinase, isozyme 1, Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial
Gene Symbol
PDK1
Additional Pyruvate Dehydrogenase Kinase 1/PDK1 Products
Product Documents for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Product Specific Notices for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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