Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunoprecipitation, Neutralization, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A8.6F10.1A6

Format

BSA Free

Concentration

1.0 mg/ml

View all QA1b Antibodies »

Product Specifications

Immunogen

Animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the Qa-1b Class Ib molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for QA1b Antibody (6A8.6F10.1A6) - BSA Free

Western Blot: QA1b Antibody (6A8.6F10.1A6)BSA Free [NBP2-26649]

Western Blot: QA1b Antibody (6A8.6F10.1A6)BSA Free [NBP2-26649]

Western Blot: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in mouse thymus tissue lysate using NBP2-26649 at 2 ug/mL.
Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) - BSA Free [NBP2-26649]

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) - BSA Free [NBP2-26649]

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. CNS cells of EAE mice stained for viable cells using APC conjugated QA1B antibody (NBP2-26649APC). Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue), and in QA1b-stained cells (red). Image from verified customer review.
Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) - BSA Free [NBP2-26649]

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) - BSA Free [NBP2-26649]

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) NBP2-26649 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in only a small percentage of cells.
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Applications for QA1b Antibody (6A8.6F10.1A6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Western Blot

1 - 2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: QA1b

Alternate Names

H2-Q1, H2-T23

Gene Symbol

QA1b

UniProt

NP_001129540

Additional QA1b Products

Product Documents for QA1b Antibody (6A8.6F10.1A6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for QA1b Antibody (6A8.6F10.1A6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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