QKI/Quaking Antibody (PCRP-QKI-2F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13817

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2C Clone # PCRP-QKI-2F10

Concentration

0.2 mg/ml

View all QKI/Quaking Antibodies »

Product Summary for QKI/Quaking Antibody (PCRP-QKI-2F10)

Immunogen

Recombinant full-length human QKI/Quaking protein (Uniprot: Q96PU8)

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2C

Theoretical MW

37.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14072)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for QKI/Quaking Antibody (PCRP-QKI-2F10)

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817]

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817]

Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817]

Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817]

Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed MCF7 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817]

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817]

Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
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Applications for QKI/Quaking Antibody (PCRP-QKI-2F10)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: concentration is assay dependent

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: QKI/Quaking

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Alternate Names

DKFZp586I0923, HKQ, homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, hqkI, protein quaking, QK, QK1, QK3, quaking homolog, KH domain RNA binding (mouse), RNA binding protein HQK

Gene Symbol

QKI

UniProt

Q96PU8 (Human)

Additional QKI/Quaking Products

Product Documents for QKI/Quaking Antibody (PCRP-QKI-2F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for QKI/Quaking Antibody (PCRP-QKI-2F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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