Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all R-Spondin 3 Antibodies »

Product Summary for R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 22-272 of human RSPO3 (NP_116173) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free

Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949]

Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949]

Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949]

Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949]

Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949]

Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949]

Immunohistochemistry: R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free [NBP2-73949] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: R-Spondin 3

The RSPO3 gene encodes a member of the thrombospondin type 1 repeat supergene family. In addition, the protein contains afurin-like cysteine-rich region. Furin-like repeat domains have been found in a variety of eukaryotic proteinsinvolved in the mechanism of

Long Name

Roof Plate-specific Spondin 3

Alternate Names

Cristin 1, PWTSR, RSPO3, RSpondin 3, THSD2

Gene Symbol

RSPO3

Additional R-Spondin 3 Products

Product Documents for R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for R-Spondin 3 Antibody (OTI3D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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