Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H4

Concentration

1 mg/ml

View all Rab1A Antibodies »

Product Summary for Rab1A Antibody (7H4)

Immunogen

Full length recombinant Rab1A protein (NP_004152.1)

Localization

Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Specificity

Detects ~23 kDa. Cross-reacts with RAB1B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rab1A Antibody (7H4)

Western Blot: Rab1A Antibody (7H4) [NBP3-11042]

Western Blot: Rab1A Antibody (7H4) [NBP3-11042]

Western Blot: Rab1A Antibody (7H4) [NBP3-11042] - Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of Rab1A protein using Mouse Anti-Rab1A Monoclonal Antibody, Clone 7H4 (NBP3-11042). Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells.. Load: 10 ug (A549, MEF); 20 ug (Mouse Brain). Primary Antibody: Mouse Anti-Rab1A Monoclonal Antibody (NBP3-11042) at 1:1000.
Immunoprecipitation: Rab1A Antibody (7H4) [NBP3-11042]

Immunoprecipitation: Rab1A Antibody (7H4) [NBP3-11042]

Immunoprecipitation: Rab1A Antibody (7H4) [NBP3-11042] - Immunoprecipitation analysis using Mouse Anti-RAB1A Monoclonal Antibody, Clone 7H4. Tissue: HEK293 cells overexpressing RAB1A, RAB1B, and RAB1C. Species: Human. Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody for 2 hours.
Rab1A Antibody (7H4)

Western Blot Shows Human Rab1A Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 human near-haploid cell line and Rab1A knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with Rab1A Antibody (Catalog # NBP3-11042) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for Rab1A (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
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Applications for Rab1A Antibody (7H4)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Rab1A

The small GTPase RAB1 controls vesicle traffic from the endoplasmic reticulum (ER) to the Golgi apparatus. Rab1 belongs to the Ras superfamily of GTPases that cycle between inactive GDP-bound and active GTP-bound forms (Allan et al., 2000 [PubMed 10903204]).[supplied by OMIM]

Long Name

Ras-related protein Rab-1A

Alternate Names

RAB1

Gene Symbol

RAB1A

Additional Rab1A Products

Product Documents for Rab1A Antibody (7H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rab1A Antibody (7H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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