Rab1A Antibody (PSH03-62)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32884
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-62
Concentration
1 mg/ml
Product Summary for Rab1A Antibody (PSH03-62)
Immunogen
Synthetic peptide within human Rab1A aa 156-205 / 205. (Uniprot: P62820)
Localization
Golgi apparatus, Endoplasmic reticulum, Early endosome, Cytoplasm, cytosol, Membrane, Melanosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rab1A Antibody (PSH03-62)
Immunohistochemistry: Rab1A Antibody (PSH03-62) [NBP3-32884]
Immunohistochemistry: Rab1A Antibody (PSH03-62) [NBP3-32884] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Rab1A antibody (NBP3-32884) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32884) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: Rab1A Antibody (PSH03-62) [NBP3-32884]
Immunocytochemistry/ Immunofluorescence: Rab1A Antibody (PSH03-62) [NBP3-32884] - Immunocytochemistry analysis of U-87 MG cells labeling Rab1A with Rabbit anti-Rab1A antibody (NBP3-32884) at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rab1A antibody (NBP3-32884) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Flow Cytometry: Rab1A Antibody (PSH03-62) [NBP3-32884]
Flow Cytometry: Rab1A Antibody (PSH03-62) [NBP3-32884] - Flow cytometric analysis of U-87 MG cells labeling Rab1A.Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32884, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Applications for Rab1A Antibody (PSH03-62)
Application
Recommended Usage
Flow Cytometry
1:1000
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rab1A
Long Name
Ras-related protein Rab-1A
Alternate Names
RAB1
Gene Symbol
RAB1A
Additional Rab1A Products
Product Documents for Rab1A Antibody (PSH03-62)
Product Specific Notices for Rab1A Antibody (PSH03-62)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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