Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all RAB1B Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the C-terminal of Human RAB1B (aa. 179-189)

Localization

Cytoplasm|Membrane|Lipid-Anchor|Cytoplasmic Side|Preautophagosomal Structure Membrane|Lipid-Anchor|Cytoplasmic Side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAB1B Antibody

Western Blot: RAB1B Antibody [NBP3-18251]

Western Blot: RAB1B Antibody [NBP3-18251]

Western Blot: RAB1B Antibody [NBP3-18251] - Western blot analysis of Rat Pancreas showing detection of 22.1 kDa RAB1B protein using Rabbit Anti-RAB1B Polyclonal Antibody (NBP3-18251). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAB1B Polyclonal Antibody (NBP3-18251) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 22.1 kDa.
Immunocytochemistry/ Immunofluorescence: RAB1B Antibody [NBP3-18251]

Immunocytochemistry/ Immunofluorescence: RAB1B Antibody [NBP3-18251]

Immunocytochemistry/Immunofluorescence: RAB1B Antibody [NBP3-18251] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAB1B Polyclonal Antibody (NBP3-18251). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RAB1B Polyclonal Antibody (NBP3-18251) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RAB1B Antibody. (D) Composite.
Immunocytochemistry/ Immunofluorescence: RAB1B Antibody [NBP3-18251]

Immunocytochemistry/ Immunofluorescence: RAB1B Antibody [NBP3-18251]

Immunocytochemistry/Immunofluorescence: RAB1B Antibody [NBP3-18251] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAB1B Polyclonal Antibody (NBP3-18251). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RAB1B Polyclonal Antibody (NBP3-18251) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RAB1B Antibody. (D) Composite.

Applications for RAB1B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAB1B

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmicsurfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential forvesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 (PubMed 9030196); Alvarez etal., 2003 (PubMed 12802079)).(supplied by OMIM)

Alternate Names

RAB1B, member RAS oncogene family, ras-related protein Rab-1B, small GTP-binding protein

Gene Symbol

RAB1B

Additional RAB1B Products

Product Documents for RAB1B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAB1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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