Rab25 Antibody (27H3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26354

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26354-100ul
NBP3-26354-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 27H3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rab25 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Rab25 [UniProt P57735]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab25 Antibody (27H3)

Rab25 Antibody (27H3)

Immunocytochemistry/Immunofluorescence: Rab25 Antibody (27H3) [NBP3-26354] -

Immunocytochemistry/Immunofluorescence: Rab25 Antibody (27H3) [NBP3-26354] - Staining of PC-3 cell with Rab25 Antibody (27H3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 516-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Rab25 Antibody (27H3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Rab25

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Long Name

RAs Genes from Brain Protein 25

Alternate Names

CATX8, RAB11C

Gene Symbol

RAB25

Additional Rab25 Products

Product Documents for Rab25 Antibody (27H3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rab25 Antibody (27H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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