Rab5a Antibody (0K5J1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15442

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15442-100ul
NBP3-15442-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0K5J1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rab5a Antibodies »

Product Summary for Rab5a Antibody (0K5J1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 116-215 of human RAB5AA (P20339). KELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab5a Antibody (0K5J1)

Western Blot: Rab5a Antibody (0K5J1) [NBP3-15442]

Western Blot: Rab5a Antibody (0K5J1) [NBP3-15442]

Western Blot: Rab5a Antibody (0K5J1) [NBP3-15442] - Western blot analysis of extracts of various cell lines, using Rab5aA Rabbit mAb (NBP3-15442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Rab5a Antibody (0K5J1)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rab5a

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes , and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5 -triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

Alternate Names

RAB5A, member RAS oncogene family, RAB5RAS-associated protein RAB5A, ras-related protein Rab-5A

Gene Symbol

RAB5A

Additional Rab5a Products

Product Documents for Rab5a Antibody (0K5J1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rab5a Antibody (0K5J1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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