Rab5a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-13253

Novus Biologicals, part of Bio-Techne
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NB120-13253
NB120-13253-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Monkey

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Rab5a Antibodies »

Product Specifications

Immunogen

Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23954637).

Localization

Cell Membrane , Endosomone , Early Endosome Membrane , Melanosome

Specificity

Detects approx 26kDa.

Marker

Early Endosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab5a Antibody

Western Blot: Rab5a Antibody [NB120-13253]

Western Blot: Rab5a Antibody [NB120-13253]

Western Blot: Rab5a Antibody [NB120-13253] - Western blot analysis of Human Cell line lysates showing detection of Rab5aprotein using Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Rab5a Antibody [NB120-13253]

Immunocytochemistry/ Immunofluorescence: Rab5a Antibody [NB120-13253]

Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NB120-13253] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x.
Immunohistochemistry: Rab5a Antibody [NB120-13253]

Immunohistochemistry: Rab5a Antibody [NB120-13253]

Immunohistochemistry: Rab5a Antibody [NB120-13253] - Immunohistochemistry analysis using Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
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Applications for Rab5a Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:80

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ul/ml of Rab5 Antibody was sufficient for detection of Rab5 in 15 ug of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary Antibody. Use in Immunoprecipitation reported in scientific literature (PMID 23954637). Use in Immunohistochemistry free floating reported in scientific literature (PMID: 31618685).

Reviewed Applications

Read 2 reviews rated 4 using NB120-13253 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab5a

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes , and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

Alternate Names

RAB5A, member RAS oncogene family, RAB5RAS-associated protein RAB5A, ras-related protein Rab-5A

Entrez Gene IDs

5868 (Human); 271457 (Mouse); 64633 (Rat)

Gene Symbol

RAB5A

UniProt

Q6FI44 (Human)

Additional Rab5a Products

Product Documents for Rab5a Antibody

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Certificate of Analysis

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Product Specific Notices for Rab5a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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