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Rab5a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20255

Novus Biologicals, part of Bio-Techne
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NBP1-20255
NBP1-20255-0.025ml

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

A synthetic peptide from the internal region of mouse Rab5a conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous in rat.

Marker

Early Endosome Marker

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Rab5a Antibody

Immunocytochemistry/ Immunofluorescence: Rab5a Antibody [NBP1-20255]

Immunocytochemistry/ Immunofluorescence: Rab5a Antibody [NBP1-20255]

Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NBP1-20255] - Mouse bone marrow derived DCs stained with anti-Rab5a antibody. ICC/IF image submitted by a verified customer review.

Applications for Rab5a Antibody

Application
Recommended Usage

Immunohistochemistry

1:300-1:2000

Immunohistochemistry-Paraffin

1:300-1:2000

Western Blot

1:300 - 1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 24554775).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-20255 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab5a

FUNCTION: Required for the fusion of plasma membranes and early endosomes.; ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome.

Alternate Names

RAB5A, member RAS oncogene family, RAB5RAS-associated protein RAB5A, ras-related protein Rab-5A

Entrez Gene IDs

271457 (Mouse); 64633 (Rat)

Gene Symbol

RAB5A

UniProt

Additional Rab5a Products

Product Documents for Rab5a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rab5a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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