Rab5a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58129

Novus Biologicals, part of Bio-Techne
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NBP2-58129
NBP2-58129-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rab5a Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: NEPQNPGANSARGRGVDLTEPTQPTRNQCCS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab5a Antibody

Western Blot: Rab5a Antibody [NBP2-58129]

Western Blot: Rab5a Antibody [NBP2-58129]

Western Blot: Rab5a Antibody [NBP2-58129] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: Rab5a Antibody [NBP2-58129]

Immunohistochemistry-Paraffin: Rab5a Antibody [NBP2-58129]

Immunohistochemistry-Paraffin: Rab5a Antibody [NBP2-58129] - Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.

Applications for Rab5a Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab5a

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes , and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5 -triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

Alternate Names

RAB5A, member RAS oncogene family, RAB5RAS-associated protein RAB5A, ras-related protein Rab-5A

Gene Symbol

RAB5A

Additional Rab5a Products

Product Documents for Rab5a Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rab5a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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