RAB6A Antibody

Western Blot: RAB6A Antibody [NBP1-33110]

Western Blot: RAB6A Antibody [NBP1-33110] - A. 50 ug rat brain lysate/extract 12 % SDS-PAGE RAB6A antibody dilution: 1:1000

Immunocytochemistry/ Immunofluorescence: RAB6A Antibody [NBP1-33110]

Immunocytochemistry/Immunofluorescence: RAB6A Antibody [NBP1-33110] - Analysis of methanol-fixed HeLa, using RAB6A antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Immunocytochemistry/ Immunofluorescence: RAB6A Antibody [NBP1-33110]

Immunocytochemistry/Immunofluorescence: RAB6A Antibody [NBP1-33110] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAB6A protein stained by RAB6A antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Knockout Validated: RAB6A Antibody [NBP1-33110]

Knockout Validated: RAB6A Antibody [NBP1-33110] - Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot Shows Human RAB6A Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 cell line and RAB6A knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with RAB6A Antibody (Catalog # NBP1-33110) followed by HRP-conjugated secondary antibody. A specific band was detected for RAB6A at approximately 24 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

RAB6A Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

HAP1 WT and RAB6A KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with RAB6A Antibody (Catalog # NBP1-33110) and with an Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1/800. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: RAB6A Antibody [NBP1-33110] -

Western Blot: RAB6A Antibody [NBP1-33110] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (NBP1-33110) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.