Rab7a Antibody (5A10A4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15444
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5A10A4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Rab7a Antibody (5A10A4)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 108-207 of human Rab7a (NP_004628.4). IQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rab7a Antibody (5A10A4)
Western Blot: Rab7a Antibody (5A10A4) [NBP3-15444]
Western Blot: Rab7a Antibody (4X3P6) [NBP3-15444] - Western blot analysis of extracts of various cell lines, using Rab7a Rabbit mAb (NBP3-15444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (5A10A4) [NBP3-15444]
Immunocytochemistry/Immunofluorescence: Rab7a Antibody (4X3P6) [NBP3-15444] - Immunofluorescence analysis of NIH-3T3 cells using Rab7a Rabbit mAb (NBP3-15444) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Rab7a Antibody (5A10A4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rab7a
Long Name
RAs Genes from Brain Protein 7a
Alternate Names
PRO2706, RAB7
Gene Symbol
RAB7A
Additional Rab7a Products
Product Documents for Rab7a Antibody (5A10A4)
Product Specific Notices for Rab7a Antibody (5A10A4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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