Rabbit TNF-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5670
Key Product Details
Species Reactivity
Rabbit
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 610331
Product Specifications
Immunogen
E. coli-derived recombinant rabbit TNF-alpha
Leu79-Leu234
Accession # NP_001075732
Leu79-Leu234
Accession # NP_001075732
Specificity
Detects rabbit TNF-alpha in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant TNF-alpha from cotton rat, cat, cow, dog, guinea pig, horse,
human, mouse, pig, rat, or rhesus is observed. No cross-reactivity with
recombinant human (rh) 4-1BB Ligand, rhAPRIL, rhBAFF, rhEDA, rhEDA-A2, rhFas
Ligand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha1 beta2, rhLymphotoxin alpha2 beta1, rhOX40
Ligand, rhTRAIL, rhTRANCE, rhTWEAK, rhVEGI, recombinant mouse (rm) 4-1BB
Ligand, rmBAFF, rmFas Ligand, rmGITR Ligand, rmLIGHT, rmOX40 Ligand, rmTRAIL,
rmTRAIL-2L, rmTRANCE, or rmTWEAK is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Rabbit TNF-alpha Antibody
Cytotoxicity Induced by TNF‑ alpha and Neutralization by Rabbit TNF‑ alpha Antibody.
Recombinant Rabbit TNF-a (Catalog # 5670-TG) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rabbit TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit TNF-a Monoclonal Antibody (Catalog # MAB5670). The ND50 is typically 0.4-2.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).Applications for Rabbit TNF-alpha Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize TNF‑ alpha-induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 0.25 ng/mL Recombinant Rabbit TNF‑ alpha and the metabolic inhibitor actinomycin D (1 µg/mL).
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF-alpha
Tumor necrosis factor (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily and family. The 26 kDa type II transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Rabbit TNF-alpha is 235 amino acids (aa) in length and contains a 35 aa cytoplasmic domain, a 21 aa transmembrane region, and a 179 extracellular domain (ECD). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. Rabbit TNF-alpha is 79% and 78% aa identical to human and mouse TNF-alpha, respectively. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.
Long Name
Tumor Necrosis Factor alpha
Alternate Names
Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2
Entrez Gene IDs
Gene Symbol
TNF
UniProt
Additional TNF-alpha Products
Product Documents for Rabbit TNF-alpha Antibody
Product Specific Notices for Rabbit TNF-alpha Antibody
For research use only
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