RABL4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87170
![Western Blot: RABL4 Antibody [NBP1-87170]](https://resources.bio-techne.com/images/products/RABL4-Antibody-Western-Blot-NBP1-87170-img0012.jpg "Western Blot: RABL4 Antibody [NBP1-87170]")
![Western Blot: RABL4 Antibody [NBP1-87170]](https://resources.bio-techne.com/images/products/RABL4-Antibody-Western-Blot-NBP1-87170-img0009.jpg "Western Blot: RABL4 Antibody [NBP1-87170]")
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TNEESFNNCSKWLEKARSQAPGISLPGVLVGNKTDLAGRRAVDSAEARAWALGQGLECFETSVKEMENFEAPFHCLAKQFHQLYREKVEV
Reactivity Notes
Rat (80%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RABL4 Antibody
![Western Blot: RABL4 Antibody [NBP1-87170]](https://resources.bio-techne.com/images/products/RABL4-Antibody-Western-Blot-NBP1-87170-img0011.jpg "Western Blot: RABL4 Antibody [NBP1-87170]")
Western Blot: RABL4 Antibody [NBP1-87170]
Western Blot: RABL4 Antibody [NBP1-87170] - IFT proteins associate with central spindle MTs in anaphase and are required for their organization. Western blots (left) showing the amount of IFT27 and IFT88 in LLC-PK1 cells transfected with control (CT), IFT27 or IFT88 siRNA. alpha-tubulin: loading control. Immunofluorescence images (right) showing a decrease of IFT88 staining in an anaphase cell upon IFT88 depletion. Scale bars: 10?um for main images, 3?um for insets. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-017-01479-3), licensed under a CC-BY license.![Western Blot: RABL4 Antibody [NBP1-87170]](https://resources.bio-techne.com/images/products/RABL4-Antibody-Western-Blot-NBP1-87170-img0010.jpg "Western Blot: RABL4 Antibody [NBP1-87170]")
Western Blot: RABL4 Antibody [NBP1-87170]
Western Blot: RABL4 Antibody [NBP1-87170] - Analysis in control (vector only transfected HEK293T lysate) and IFT27 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Applications for RABL4 Antibody
Application
Recommended Usage
Western Blot
0.04-0.4 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RABL4
Alternate Names
intraflagellar transport 27 homolog (Chlamydomonas), intraflagellar transport protein 27 homolog, member of RAS oncogene family-like 4, Putative GTP-binding protein RAY-like, RABL4, Rab-like protein 4
Gene Symbol
IFT27
Additional RABL4 Products
Product Documents for RABL4 Antibody
Product Specific Notices for RABL4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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