Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CPTC-RAC1-1

Concentration

0.2 mg/ml

View all Rac1 Antibodies »

Product Specifications

Immunogen

Recombinant human full-length Rac1protein (Uniprot: P63000)

Localization

Cell surface, Melanosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08939)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Rac1 Antibody (CPTC-RAC1-1)

Immunohistochemistry-Paraffin: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195]

Immunohistochemistry-Paraffin: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195]

Immunohistochemistry-Paraffin: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195] - Formalin-fixed, paraffin-embedded human lung stained with Rac1 Mouse Monoclonal Antibody (CPTC-Rac1-1).
Immunohistochemistry-Paraffin: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195]

Immunohistochemistry-Paraffin: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195]

Immunohistochemistry-Paraffin: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195] - Formalin-fixed, paraffin-embedded human lung stained with Rac1 Mouse Monoclonal Antibody (CPTC-Rac1-1).
Protein Array: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195]

Protein Array: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195]

Protein Array: Rac1 Antibody (CPTC-RAC1-1) [NBP3-07195] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Rac1Monoclonal Antibody (CPTC-RAC1-1).

Applications for Rac1 Antibody (CPTC-RAC1-1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Rac1

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Long Name

Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, small GTP binding Protein Rac1)

Alternate Names

p21-Rac1, TC-25

Gene Symbol

RAC1

UniProt

P63000 (Human)

Additional Rac1 Products

Product Documents for Rac1 Antibody (CPTC-RAC1-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rac1 Antibody (CPTC-RAC1-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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